Prunus persica transcription factor PpNAC56 enhances heat resistance in transgenic tomatoes

Xiangguang Meng; Ning Wang; Huajie He; Qiuping Tan; Binbin Wen; Rui Zhang; Xiling Fu; Wei Xiao; Xiude Chen; Dongmei Li; Ling Li

doi:10.1016/j.plaphy.2022.04.026

Prunus persica transcription factor PpNAC56 enhances heat resistance in transgenic tomatoes

Plant Physiol Biochem. 2022 Jul 1:182:194-201. doi: 10.1016/j.plaphy.2022.04.026. Epub 2022 Apr 30.

Authors

Xiangguang Meng¹, Ning Wang¹, Huajie He¹, Qiuping Tan¹, Binbin Wen¹, Rui Zhang¹, Xiling Fu¹, Wei Xiao¹, Xiude Chen¹, Dongmei Li², Ling Li³

Affiliations

¹ College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, PR China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, PR China; Shandong Province Collaborative Innovation Center for High-quality and High-efficiency Vegetable Production, Tai'an, 271018, PR China.
² College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, PR China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, PR China; Shandong Province Collaborative Innovation Center for High-quality and High-efficiency Vegetable Production, Tai'an, 271018, PR China. Electronic address: [email protected].
³ College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, PR China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, PR China; Shandong Province Collaborative Innovation Center for High-quality and High-efficiency Vegetable Production, Tai'an, 271018, PR China. Electronic address: [email protected].

PMID: 35525200
DOI: 10.1016/j.plaphy.2022.04.026

Abstract

Members of the NAC (NAM, ATAF1,2 and CUC2) transcription factor family are involved in numerous processes of plant growth and development and play an important role in the response to abiotic stresses such as salinity, drought and heat, but little research on this topic has been done in peach. In this study, we analyzed the expression patterns of PpNAC56 under abiotic stress and found that PpNAC56 responded to high-temperature stress. To verify the function of PpNAC56, we overexpressed this gene in tomato plants and found that, compared with WT plants, the transgenic tomato plants could accumulate more osmoregulatory substances after high-temperature treatment and thus were more heat resistance. Then, using Y2H, BIFC, and pull-down assays, we found that PpNAC56 could interact with PpMIEL1. In addition, Y1H and dual-luciferase assays verified that PpNAC56 could activate the expression of PpHSP17.4 and PpSnRK2D. The above experimental results demonstrate that PpNAC56 plays an important role in the plant response to heat stress.

Keywords: Heat stress; Peach; PpMIEL1; PpNAC56.

MeSH terms

Arabidopsis* / genetics
Droughts
Gene Expression Regulation, Plant
Plant Proteins / genetics
Plant Proteins / metabolism
Plants, Genetically Modified / metabolism
Prunus persica* / metabolism
Solanum lycopersicum* / genetics
Solanum lycopersicum* / metabolism
Stress, Physiological / genetics
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Plant Proteins
Transcription Factors