Evaluation of trefoil factor 3 as a non-invasive biomarker of gastric intestinal metaplasia and gastric cancer in a high-risk population

Gastroenterol Hepatol. 2023 Jun-Jul;46(6):411-418. doi: 10.1016/j.gastrohep.2022.04.004. Epub 2022 May 14.
[Article in English, Spanish]

Abstract

Background: Adenocarcinoma is preceded by chronic atrophic gastritis, gastric intestinal metaplasia and dysplasia. Trefoil factor 3 (TFF3) is a peptide secreted by goblet cells, which is abundantly present in intestinal metaplasia.

Aim: To evaluate the utility of serum TFF3 as a non-invasive biomarker for the diagnosis of intestinal metaplasia and gastric cancer.

Methods: Single-center, cross-sectional study of 274 patients who consecutively underwent upper gastrointestinal endoscopy with gastric biopsies (updated Sydney system). TFF3 levels were measured in serum by a commercial ELISA kit. Patients with normal histology or chronic atrophic gastritis without intestinal metaplasia comprised the control group. In addition, 14 patients with invasive gastric cancer were included as a reference group. The association between TFF3 levels and intestinal metaplasia was assessed by logistic regression.

Results: Patients with intestinal metaplasia (n=110) had a higher median TFF3 level as compared to controls (n=164), 13.1 vs. 11.9ng/mL, respectively (p=0.024). Multivariable logistic regression showed a no significant association between TFF3 levels and intestinal metaplasia (OR=1.20; 95%CI: 0.87-1.65; p-trend=0.273). The gastric cancer group had a median TFF3 level of 20.5ng/mL, and a significant association was found (OR=3.26; 95%CI: 1.29-8.27; p-trend=0.013).

Conclusion: Serum levels of TFF3 do not discriminate intestinal metaplasia in this high-risk Latin American population. Nevertheless, we confirmed an association between TFF3 levels and invasive gastric cancer.

Keywords: Condiciones premalignas; Cáncer gástrico; Gastric cancer; Intestinal metaplasia; Metaplasia intestinal; Precancerous conditions; Trefoil factor 3.

MeSH terms

  • Biomarkers
  • Cross-Sectional Studies
  • Gastric Mucosa
  • Gastritis, Atrophic*
  • Helicobacter pylori*
  • Humans
  • Metaplasia / pathology
  • Precancerous Conditions* / pathology
  • Stomach Neoplasms* / pathology
  • Trefoil Factor-3

Substances

  • Trefoil Factor-3
  • Biomarkers