Abstract
Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the phosphatidyl residue from phosphatidylcholine to the 5'-hydroxyl group of nucleosides. A variety of 5'-phosphatidylnucleosides were prepared by this reaction in high yields. Some of them showed marked antitumor activities in mice.
MeSH terms
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Animals
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Antimetabolites, Antineoplastic / chemical synthesis*
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Leukemia P388 / drug therapy
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Mice
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Nucleotides / chemical synthesis*
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Nucleotides / therapeutic use
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Phosphatidic Acids / chemical synthesis*
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Phosphatidic Acids / therapeutic use
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Phospholipase D / metabolism*
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Phospholipases / metabolism*
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Streptomyces / enzymology
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Structure-Activity Relationship
Substances
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Antimetabolites, Antineoplastic
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Nucleotides
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Phosphatidic Acids
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Phospholipases
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Phospholipase D