Peroxidase-Mediated In Situ Fabrication of Multi-Stimuli-Responsive and Dynamic Protein Nanogels from Tyrosine-Conjugated Biodynamer and Ovablumin

Lin Wang; Li Liu; Bingyang Dong; Hanying Zhao

doi:10.1021/acsmacrolett.9b00465

Peroxidase-Mediated In Situ Fabrication of Multi-Stimuli-Responsive and Dynamic Protein Nanogels from Tyrosine-Conjugated Biodynamer and Ovablumin

ACS Macro Lett. 2019 Oct 15;8(10):1233-1239. doi: 10.1021/acsmacrolett.9b00465. Epub 2019 Sep 13.

Authors

Lin Wang^{1

2}, Li Liu¹, Bingyang Dong¹, Hanying Zhao¹

Affiliations

¹ Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, People's Republic of China.
² School of Packaging and Printing Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, People's Republic of China.

PMID: 35651157
DOI: 10.1021/acsmacrolett.9b00465

Abstract

Horseradish peroxidase (HRP)-mediated oxidation of tyrosine-conjugated biodynamer containing acylhydrazone linkages and ovalbumin (OVA)/reduced ovalbumin (rOVA) generated protein nanogels through simultaneous tyrosine coupling and thiol cross-linking in the presence of H₂O₂. The obtained nanogels are multi-stimuli-responsive to temperature, pH, and glutathione (GSH) and possess the dynamic character of reversible covalent bonds. The OVA-based or rOVA-based protein nanogels can be utilized as cargoes of anticancer drug curcumin (Cur). The Cur-loaded protein nanogels rapidly released Cur in intracellular-mimicking acidic and reductive environment, thus, making these protein nanogels promising nanocarriers for intracellular drug delivery. HRP-mediated cross-linking of the tyrosine-conjugated biodynamer and proteins provides a facile and versatile approach for fabrication of responsive and adaptive biohybrid nanogels under mild conditions.