Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells

Elife. 2022 Jun 6:11:e74443. doi: 10.7554/eLife.74443.

Abstract

COVID-19 is a disease of dysfunctional immune responses, but the mechanisms triggering immunopathogenesis are not established. The functional plasticity of macrophages allows this cell type to promote pathogen elimination and inflammation or suppress inflammation and promote tissue remodeling and injury repair. During an infection, the clearance of dead and dying cells, a process named efferocytosis, can modulate the interplay between these contrasting functions. Here, we show that engulfment of SARS-CoV-2-infected apoptotic cells exacerbates inflammatory cytokine production, inhibits the expression of efferocytic receptors, and impairs continual efferocytosis by macrophages. We also provide evidence supporting that lung monocytes and macrophages from severe COVID-19 patients have compromised efferocytic capacity. Our findings reveal that dysfunctional efferocytosis of SARS-CoV-2-infected cell corpses suppresses macrophage anti-inflammation and efficient tissue repair programs and provides mechanistic insights for the excessive production of pro-inflammatory cytokines and accumulation of tissue damage associated with COVID-19 immunopathogenesis.

Keywords: COVID-19; SARS-CoV-2; efferocytosis; human; hyperinflammation; immunology; inflammation; macrophage polarization; tissue repair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology
  • Apoptosis
  • COVID-19*
  • Humans
  • Macrophages / metabolism
  • Phagocytosis
  • SARS-CoV-2*

Substances

  • Anti-Inflammatory Agents

Associated data

  • GEO/GSE145926
  • GEO/GSE158055

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.