Molecular Tools for Candida auris Identification and Typing

Auke W de Jong; Bert Gerrits van den Ende; Ferry Hagen

doi:10.1007/978-1-0716-2417-3_3

Molecular Tools for Candida auris Identification and Typing

Methods Mol Biol. 2022:2517:33-41. doi: 10.1007/978-1-0716-2417-3_3.

Authors

Auke W de Jong^{1

2}, Bert Gerrits van den Ende¹, Ferry Hagen^{3

4

5}

Affiliations

¹ Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
² Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
³ Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands. [email protected].
⁴ Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands. [email protected].
⁵ Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands. [email protected].

PMID: 35674943
DOI: 10.1007/978-1-0716-2417-3_3

Abstract

Identification of Candida auris by conventional identification methodologies can be challenging. While whole genome sequencing is seen as the golden standard to genotype C. auris at an inter- and intraspecies level, it is costly and time-consuming. Sequencing the transcribed spacer (ITS) region and microsatellite typing provide simple, fast, and inexpensive alternatives for identification and genotyping of C. auris. Here we will describe both molecular approaches.

Keywords: Candida auris; Genotyping; ITS sequencing; Microsatellite typing; Molecular epidemiology.

MeSH terms

Antifungal Agents
Candida auris
Candida* / genetics
Candidiasis* / diagnosis
Genotype
Humans
Microbial Sensitivity Tests

Substances

Antifungal Agents