Evaluation of Resveratrol and Piceatannol Anticonvulsant Potential in Adult Zebrafish (Danio rerio)

Neurochem Res. 2022 Nov;47(11):3250-3260. doi: 10.1007/s11064-022-03656-3. Epub 2022 Jun 24.

Abstract

Epilepsy is a common neurological disorder which affects 50 million people worldwide. Patients with epilepsy may present cognitive deficits and psychological impairment. Currently, 30% of patients fail to respond to any available antiseizure drug, and a significant number of patients do not well tolerate the offered treatments. Then, it is necessary to find out alternatives for controlling epileptic seizures. Studies have shown that despite its neuroprotective effects, resveratrol shows poor anticonvulsant properties. Resveratrol analog, piceatannol, possesses higher biological activity than resveratrol and could be an alternative to control seizure. Thus, the present study investigated the effects of resveratrol and piceatannol in pentylenetetrazole-induced seizures in adult zebrafish (Danio rerio). Only the experimental positive control (diazepam) showed anticonvulsant effect in this study. In addition, no behavioral changes were observed 24 h after seizure occurrence. Finally, the expression of genes related to neuronal activity (c-fos), neurogenesis (p70S6Ka and p70S6Kb), inflammatory response (interleukin 1β), and cell apoptosis (caspase-3) did not change by pentylenetetrazole-induced seizures. Therefore, we failed to observe any anticonvulsant and neuroprotective potential of resveratrol and piceatannol in adult zebrafish. However, resveratrol and piceatannol benefits in epilepsy are not discharged, and more studies are necessary.

Keywords: Epilepsy; Piceatannol; Resveratrol; Seizure; Zebrafish.

MeSH terms

  • Animals
  • Anticonvulsants / adverse effects
  • Caspase 3
  • Diazepam / therapeutic use
  • Epilepsy* / drug therapy
  • Interleukin-1beta
  • Neuroprotective Agents* / adverse effects
  • Pentylenetetrazole / toxicity
  • Resveratrol / pharmacology
  • Resveratrol / therapeutic use
  • Seizures / chemically induced
  • Seizures / drug therapy
  • Stilbenes
  • Zebrafish

Substances

  • Anticonvulsants
  • Interleukin-1beta
  • Neuroprotective Agents
  • Stilbenes
  • 3,3',4,5'-tetrahydroxystilbene
  • Caspase 3
  • Resveratrol
  • Diazepam
  • Pentylenetetrazole