The internalization mechanisms and trafficking of the pea albumin in Caco-2 cells

Weibo Zhang; Shucheng Zhang; Chong Chen; Ning Liu; Dong Yang; Pengjie Wang; Fazheng Ren

doi:10.1016/j.ijbiomac.2022.06.149

The internalization mechanisms and trafficking of the pea albumin in Caco-2 cells

Int J Biol Macromol. 2022 Sep 30:217:111-119. doi: 10.1016/j.ijbiomac.2022.06.149. Epub 2022 Jun 25.

Authors

Weibo Zhang¹, Shucheng Zhang², Chong Chen³, Ning Liu⁴, Dong Yang⁵, Pengjie Wang⁶, Fazheng Ren⁷

Affiliations

¹ Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.. Electronic address: [email protected].
² Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
³ Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: [email protected].
⁴ Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
⁵ Inner Mongolia Caoyuanxinhe Technology Research Co. Ltd., Inner Mongolia 01500, China.
⁶ Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.. Electronic address: [email protected].
⁷ Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.. Electronic address: [email protected].

PMID: 35764167
DOI: 10.1016/j.ijbiomac.2022.06.149

Abstract

Pea albumin (PA) can reach the intestine in the active form because it is highly resistant to gastric acid and proteolytic enzymes after their oral intake, which can supply various bioactivities. However, there is no detailed knowledge of the intestinal cell uptake about PA. The aim of this work was to study the internalization mechanism and intracellular trafficking route of PA. The uptake of PA-cyanine 5.5 NHS ester (Cy5.5) was a time-dependent and concentration-dependent process in Caco-2 cells. Endocytosis inhibitors or small interfering RNA (siRNA) techniques revealed that the internalization of PA-Cy5.5 was energy-dependent and mediated by caveolin-mediated endocytosis. Furthermore, we observed colocalization of PA-Cy5.5 and its subcellular localization in Caco-2 cells by using confocal laser scanning microscopy, which revealed that the intracellular trafficking process of PA-Cy5.5 was related to endoplasmic reticulum, Golgi, and lysosome. Interestingly, PA can alleviate lipopolysaccharide -induced ER stress, which may be the main reason why pea albumin is anti-inflammatory. Overall, our findings suggest caveolin may be critical for PA uptake in enterocytes and could contribute to explore the bioactivities mechanism of pea albumin in body.

Keywords: Caveolin; Endoplasmic reticulum; Internalization of pea albumin.

MeSH terms

Albumins
Caco-2 Cells
Caveolins
Endocytosis*
Humans
Pisum sativum*
RNA, Small Interfering

Substances

Albumins
Caveolins
RNA, Small Interfering