Protocol for evaluating CD8+ T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

STAR Protoc. 2022 Sep 16;3(3):101479. doi: 10.1016/j.xpro.2022.101479. Epub 2022 Jun 22.

Abstract

Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8+ T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIVSF162P3-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8+ T cells in SVC macaques. For complete details on the use and execution of this protocol, please refer to Xue et al. (2022).

Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Microbiology; Model Organisms; Molecular Biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes
  • Chemokines
  • Cytokines
  • HIV Infections* / drug therapy
  • Immunity, Cellular
  • Lipopeptides / pharmacology
  • Macaca mulatta
  • Simian Acquired Immunodeficiency Syndrome* / drug therapy
  • Simian Immunodeficiency Virus*
  • Viral Load

Substances

  • Chemokines
  • Cytokines
  • Lipopeptides