A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

STAR Protoc. 2022 Sep 16;3(3):101489. doi: 10.1016/j.xpro.2022.101489. Epub 2022 Jun 23.

Abstract

Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).

Keywords: Metabolism; Molecular Biology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Arginine / metabolism
  • Autophagy*
  • Energy Metabolism
  • Humans
  • Hydrolases* / metabolism
  • Mice
  • Polyethylene Glycols

Substances

  • Polyethylene Glycols
  • Arginine
  • Hydrolases
  • ADI PEG20