Abstract
I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Ampicillin*
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Base Sequence
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Escherichia coli / genetics*
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Genes*
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Penicillin Resistance*
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Plasmids*
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beta-Lactamases / genetics
Substances
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Ampicillin
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beta-Lactamases
Associated data
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GENBANK/J01749
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GENBANK/J01832
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GENBANK/K00005
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GENBANK/M10282
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GENBANK/M10283
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GENBANK/M10286
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GENBANK/M10356
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GENBANK/M10784
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GENBANK/M10785
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GENBANK/M10786