Antibacterial Activity Evaluation of ZnO, CuO, and TiO2 Nanoparticles in Solution and Thin Films

Christine Mielcarek; Rania Dadi; Anne Roynette; Alex Lemarchand; Andrei Kanaev; Karim Senni; Mamadou Traore; Rabah Azouani

doi:10.1007/978-1-0716-2473-9_4

Antibacterial Activity Evaluation of ZnO, CuO, and TiO₂ Nanoparticles in Solution and Thin Films

Methods Mol Biol. 2022:2525:35-46. doi: 10.1007/978-1-0716-2473-9_4.

Authors

Christine Mielcarek¹, Rania Dadi², Anne Roynette¹, Alex Lemarchand², Andrei Kanaev², Karim Senni¹, Mamadou Traore², Rabah Azouani³

Affiliations

¹ EBI-Ecole de Biologie Industrielle, Cergy, France.
² LSPM-CNRS, Laboratoire des Sciences des Procédés et des Matériaux, Université Paris 13, Sorbonne Paris Cité, Villetaneuse, France.
³ EBI-Ecole de Biologie Industrielle, Cergy, France. [email protected].

PMID: 35836059
DOI: 10.1007/978-1-0716-2473-9_4

Abstract

This chapter introduces unique methodology of antibacterial activity evaluation of nanoparticles in both solution and thin films. Nanoparticles of ZnO, TiO₂, and CuO are synthesized via the sol-gel method. Antibacterial tests are carried out against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria using disk diffusion and bioluminescence. To perform antibacterial tests on thin films and to overcome bacterial strains recuperation on the supports, a new method of bacterial detaching from the slides is developed based on French standard NF EN 14561.

Keywords: ATP bioluminescence; Antibacterial; CuO; Disk diffusion method; TiO2 nanoparticles; ZnO.

MeSH terms

Anti-Bacterial Agents / pharmacology
Copper
Escherichia coli
Gram-Negative Bacteria
Microbial Sensitivity Tests
Nanoparticles*
Titanium
Zinc Oxide* / pharmacology

Substances

Anti-Bacterial Agents
titanium dioxide
Copper
Titanium
Zinc Oxide
cupric oxide