HDAC11 Regulates the Proliferation of Bovine Muscle Stem Cells through the Notch Signaling Pathway and Inhibits Muscle Regeneration

J Agric Food Chem. 2022 Jul 27;70(29):9166-9178. doi: 10.1021/acs.jafc.2c03384. Epub 2022 Jul 15.

Abstract

Myogenesis is an essential process that can affect the yield and quality of beef. Transcriptional studies have shown that histone deacetylase 11 (HDAC11) was differentially expressed in muscle tissues of 6 and 18 month old Longlin cattle, but its role in the regulation of myogenesis remains unclear. This study aimed to determine the role of HDAC11 in the proliferation and differentiation of bovine muscle stem cells (MuSCs). HDAC11 promoted MuSC proliferation by activating Notch signaling and inhibited myoblast differentiation by reducing MyoD1 transcription. In addition, overexpression of HDAC11 inhibited the repair regeneration process of muscle in mice. HDAC11 was found to be a novel key target for the control of myogenesis, and this is a theoretical basis for the development of HDAC11-specific modulators as a new strategy to regulate myogenesis.

Keywords: HDAC11; Longlin cattle; Notch signaling pathway; cell proliferation; longissimus dorsi; muscle stem cells.

MeSH terms

  • Animals
  • Cattle
  • Cell Differentiation
  • Cell Proliferation
  • Histone Deacetylases* / genetics
  • Histone Deacetylases* / metabolism
  • Mice
  • Muscle, Skeletal / metabolism
  • Muscles / metabolism
  • Myoblasts*
  • Regeneration / genetics
  • Signal Transduction

Substances

  • Hdac11 protein, mouse
  • Histone Deacetylases