Correcting for the hemoglobin absorption artifact in fiber photometry data

Run Zhang; Christina K Kim

doi:10.1016/j.crmeth.2022.100257

Correcting for the hemoglobin absorption artifact in fiber photometry data

Cell Rep Methods. 2022 Jul 18;2(7):100257. doi: 10.1016/j.crmeth.2022.100257.

Authors

Run Zhang¹, Christina K Kim²

Affiliations

¹ Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
² Department of Neurology, Center for Neuroscience, University of California, Davis, Davis, CA, USA.

Abstract

Fiber photometry is a widely used fluorescence approach for measuring neuronal activity in freely behaving animals; however, these signals can be contaminated by hemoglobin absorption artifacts. Zhang et al. propose a computational method to quantify and correct hemoglobin photon absorption effects using spectral fiber photometry, resulting in more accurate neuronal activity measurements.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

Animals
Artifacts*
Neurons / physiology
Photometry* / methods