Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates

Bismarck Dinko; Richmond Tackie; Gisela Henriques; Lindsay Stewart; Colin Sutherland

doi:10.1007/978-1-0716-2189-9_11

Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates

Methods Mol Biol. 2022:2470:121-132. doi: 10.1007/978-1-0716-2189-9_11.

Authors

Bismarck Dinko¹, Richmond Tackie², Gisela Henriques³, Lindsay Stewart³, Colin Sutherland³

Affiliations

¹ Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana. [email protected].
² Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana.
³ Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.

PMID: 35881343
DOI: 10.1007/978-1-0716-2189-9_11

Abstract

Reproducible induction of gametocytes of Plasmodium falciparum in vitro is crucial for performing various experimental analyses to understand gametocyte cellular and molecular biology and immunology, and for the evaluation of antigametocidal agents and vaccine development. In this protocol, we present specific procedures for the enrichment, synchronous production and separation of developmental stages of P. falciparum gametocytes from culture-adapted field isolates.

Keywords: Clinical/Field isolates; Gametocytes; MACS purification and enrichment; P. falciparum; Stage-specific purification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Malaria, Falciparum* / prevention & control
Plasmodium falciparum*

Grants and funding

110090/Z/15/Z/WT_/Wellcome Trust/United Kingdom