Effect of dose-rate on the survival of murine spermatogonia following 60Co gamma-irradiation

Radiother Oncol. 1987 Apr;8(4):345-51. doi: 10.1016/s0167-8140(87)80185-8.

Abstract

The survival of murine clonogenic stem and differentiated spermatogonia was assessed by counts of repopulating tubules in histological sections at 35 days and counts of testicular sperm-heads at 29 days respectively, following irradiation delivered at five dose-rates (1.1, 2.5, 5, 11 and 112 Cgy X min-1). For stem spermatogonia, a significant increase in cell survival accompanied the reduction in dose-rate, and this was characterized by an increase of the Do for the derived survival curves from 2.86 +/- 0.29 Gy at 112 cGy X min-1 to 4.46 +/- 0.45 Gy at 1.1 cGy X min-1. The data were analysed using two mathematical models for repair at varying dose-rates, the LPL model of Curtis and the incomplete repair model of Thames. Using these models the half-time for repair was estimated to lie between 15 and 25 min. No dose-rate effects were observed for the differentiated spermatogonia. The sparing of stem spermatogonia at reduced dose-rate, may have implications for the future fertility of men treated with low dose-rate total body irradiation (TBI).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Male
  • Mice
  • Mice, Inbred CBA
  • Spermatogonia / radiation effects*
  • Spermatozoa / radiation effects*
  • Stem Cells / radiation effects