PavGA2ox-2L inhibits the plant growth and development interacting with PavDWARF in sweet cherry (Prunus avium L.)

Xunju Liu; Jiyuan Wang; Irfan Ali Sabir; Wanxia Sun; Li Wang; Yan Xu; Niangong Zhang; Haobo Liu; Songtao Jiu; Lu Liu; Caixi Zhang

doi:10.1016/j.plaphy.2022.07.019

PavGA2ox-2L inhibits the plant growth and development interacting with PavDWARF in sweet cherry (Prunus avium L.)

Plant Physiol Biochem. 2022 Sep 1:186:299-309. doi: 10.1016/j.plaphy.2022.07.019. Epub 2022 Jul 21.

Authors

Xunju Liu¹, Jiyuan Wang², Irfan Ali Sabir³, Wanxia Sun⁴, Li Wang⁵, Yan Xu⁶, Niangong Zhang⁷, Haobo Liu⁸, Songtao Jiu⁹, Lu Liu¹⁰, Caixi Zhang¹¹

Affiliations

¹ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
² Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
³ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁴ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁵ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁶ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁷ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁸ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
⁹ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
¹⁰ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].
¹¹ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China. Electronic address: [email protected].

PMID: 35932654
DOI: 10.1016/j.plaphy.2022.07.019

Abstract

Dwarf dense planting is helpful to improve the yield and quality of sweet cherry, which has enormous market demand. GA2oxs (GA oxidases) affect plant height, dormancy release, flower development, and seed germination by participating in the metabolic regulation and signal transduction of GA (Gibberellin). However, the research on GA2ox in sweet cherry is little and worthy of further investigation. Therefore, we identified the PavGA2ox-2L gene from sweet cherry, close to PynGA2ox-2 from Prunus yedoensis var. Nudiflora. The phylogenetic analysis indicated conserved functions with these evolutionarily closer GA2ox subfamily genes. Subcellular localization forecast analysis indicated that PavGA2ox-2L was localized in the nucleus or cytoplasm. The expression levels of PavGA2ox-2L were higher in winter, indicating that PavGA2ox-2L promoted maintained flower bud dormancy. The expression levels of PavGA2ox-2L were significantly increased after GA₄₊₇ treatment while decreased after GR24 (a synthetic analog of SLs (Strigolactones)) or TIS108 (a triazole-type SL-biosynthesis inhibitor) treatments. Over-expression of PavGA2ox-2L resulted in decreased plant height, delayed flowering time, and low seed germination rate in Arabidopsis thaliana. Furthermore, the interaction between PavGA2ox-2L and PavDWARF was verified by Y2H and BiFC assays. In the current investigation, PavGA2ox-2L functions as a GA metabolic gene that promotes dwarf dense planting, delays flowering time, and inhibits seed germination. In addition, it also participates in regulating plant growth and development through the interaction with the critical negative regulator PavDWARF of Gibberellin. These results will help us better explore the molecular mechanism of GA2ox-mediated dwarf and late-maturing varieties for fruit trees.

Keywords: Dwarf; Flowering; PavGA2ox-2L; Seed germination; Sweet cherry.

MeSH terms

Arabidopsis* / genetics
Gene Expression Regulation, Plant
Gibberellins / metabolism
Phylogeny
Plant Development
Prunus avium* / metabolism

Substances

Gibberellins