The complete chloroplast genome of Agave amaniensis (Asparagales: Asparagaceae: Agavoideae)

Mitochondrial DNA B Resour. 2022 Aug 22;7(8):1519-1521. doi: 10.1080/23802359.2022.2109440. eCollection 2022.

Abstract

Agave amaniensis Trel. & W. Nowell (1933) has long been used for phytosteroid production, which is also one of the parents of the famous Agave hybrid cultivar 11648 for sisal fiber production. However, its systematic position and phylogenetic relationship remains unknown at the chloroplast (cp) genome level. Therefore, we have sequenced and assembled the cp genome of A. amaniensis via Illumina sequencing. The cp genome is 157,282 bp in length with a GC content of 37.84%. A large single-copy region of 85,899 bp, a small single-copy region of 18,233 bp, and inverted repeat regions of 26,575 bp were found in the cp genome. Based on the annotation, 86 protein-coding genes, eight rRNAs, and 38 tRNAs were identified in the cp genome with total lengths of 78,981 bp, 9050 bp, and 2867 bp, respectively. The phylogenetic tree indicates that A. amaniensis is closely related with A. H11648, A. angustifolia, and A. americana.

Keywords: Agave amaniensis; chloroplast genome; phylogenetic tree.

Grants and funding

This research was supported by Hainan Provincial Natural Science Foundation of China [322MS112], China Agriculture Research System of MOF and MARA [CARS-16], Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences [1630042022005], Guangxi Key R&D Program [GuiKe-AB20297007], Fundamental Research Fund of Guangxi Academy of Agricultural Sciences [Guinongke-2021YT153], and the Innovation Platform for Academicians of Hainan Province.