Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil

Arch Virol. 2022 Dec;167(12):2817-2820. doi: 10.1007/s00705-022-05594-5. Epub 2022 Sep 20.

Abstract

A cytorhabdovirus, tentatively named "patchouli chlorosis-associated cytorhabdovirus" (PCaCV), was identified in a patchouli plant, using high-throughput sequencing, and its genome sequence was confirmed by Sanger sequencing. The PCaCV genome consists of 12,913 nucleotides and contains six open reading frames in the order 3'-N-P'-P-P3-M-(G)-L-5'. The glycoprotein gene was found to contain stop codons in the coding frame; hence, this gene is considered defective. PCaCV is most closely related to tomato yellow mottle-associated virus, sharing 61.1% nucleotide sequence identity in the complete genome and 73.9% amino acid sequence identity in the L protein. These data suggest that PCaCV should be considered a new member of the genus Cytorhabdovirus, and the binomial species name "Cytorhabdovirus patchoulii" is proposed.

MeSH terms

  • Begomovirus* / genetics
  • Genome, Viral
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases
  • Pogostemon* / genetics
  • RNA, Viral / genetics
  • Rhabdoviridae* / genetics

Substances

  • RNA, Viral