Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells

Lavinija Matakovic; Arend W Overeem; Karin Klappe; Sven C D van IJzendoorn

doi:10.1007/978-1-0716-2557-6_4

Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells

Methods Mol Biol. 2022:2544:71-82. doi: 10.1007/978-1-0716-2557-6_4.

Authors

Lavinija Matakovic¹, Arend W Overeem^{1

2}, Karin Klappe¹, Sven C D van IJzendoorn³

Affiliations

¹ Department of Biomedical Sciences of Cells and Systems, section Molecular Cell Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
² Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, the Netherlands.
³ Department of Biomedical Sciences of Cells and Systems, section Molecular Cell Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands. [email protected].

PMID: 36125710
DOI: 10.1007/978-1-0716-2557-6_4

Abstract

Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.

Keywords: Bile canaliculus; Hepatocyte; Hepatocyte polarity; Induced pluripotent stem cell; Inherited liver disease.

MeSH terms

Bile Canaliculi* / metabolism
Cells, Cultured
Hepatocytes / metabolism
Humans
Mutant Proteins / metabolism
Pluripotent Stem Cells* / metabolism

Substances

Mutant Proteins