The effect of inosine on the spectroscopic properties and crystal structure of a NIR-emitting DNA-stabilized silver nanocluster

Cecilia Cerretani; Mikkel B Liisberg; Vanessa Rück; Jiro Kondo; Tom Vosch

doi:10.1039/d2na00325b

The effect of inosine on the spectroscopic properties and crystal structure of a NIR-emitting DNA-stabilized silver nanocluster

Nanoscale Adv. 2022 Jun 20;4(15):3212-3217. doi: 10.1039/d2na00325b. eCollection 2022 Jul 29.

Authors

Cecilia Cerretani¹, Mikkel B Liisberg¹, Vanessa Rück¹, Jiro Kondo², Tom Vosch¹

Affiliations

¹ Nanoscience Center and Department of Chemistry, University of Copenhagen Universitetsparken 5 2100 Copenhagen Denmark [email protected].
² Department of Materials and Life Sciences, Sophia University 7-1 Kioi-cho Chiyoda-ku 102-8554 Tokyo Japan [email protected].

Abstract

The effect of replacing guanosines with inosines in the two stabilizing strands (5'-CACCTAGCGA-3') of the NIR emissive DNA-Ag₁₆NC was investigated. The spectroscopic behavior of the inosine mutants is position-dependent: when the guanosine in position 7 was exchanged, the nanosecond fluorescence decay time shortened, while having the inosine in position 9 made the decay time longer. Thanks to structural information gained from single crystal X-ray diffraction measurements, it was possible to propose a mechanistic origin for the observed changes.