Targeted Manipulation of Histone Modification in Medaka Embryos

Hiroto S Fukushima; Hiroyuki Takeda; Ryohei Nakamura

doi:10.1007/978-1-0716-2724-2_20

Targeted Manipulation of Histone Modification in Medaka Embryos

Methods Mol Biol. 2023:2577:279-293. doi: 10.1007/978-1-0716-2724-2_20.

Authors

Hiroto S Fukushima¹, Hiroyuki Takeda¹, Ryohei Nakamura²

Affiliations

¹ Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
² Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan. [email protected].

PMID: 36173581
DOI: 10.1007/978-1-0716-2724-2_20

Abstract

Recent development of targeted manipulation of histone modification enables us to experimentally and directly test the functional relevance of histone modifications accumulated at specific genomic regions. In particular, dCas9 epigenome editing has been widely used for site-specific manipulation of epigenetic modification. Here, we describe how to apply dCas9 epigenome editing in fish (medaka, Oryzias latipes) embryos and how to analyze induced changes in histone modification.

Keywords: ChIP; Chromatin; Epigenome editing; Fish; H3K27me3; Histone modification; In vivo; Medaka.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Editing
Histone Code
Histones / genetics
Histones / metabolism
Oryzias* / genetics
Oryzias* / metabolism
Protein Processing, Post-Translational

Substances

Histones