The gut peptide Reg3g links the small intestine microbiome to the regulation of energy balance, glucose levels, and gut function

Cell Metab. 2022 Nov 1;34(11):1765-1778.e6. doi: 10.1016/j.cmet.2022.09.024. Epub 2022 Oct 13.

Abstract

Changing composition of the gut microbiome is an important component of the gut adaptation to various environments, which have been implicated in various metabolic diseases including obesity and type 2 diabetes, but the mechanisms by which the microbiota influence host physiology remain contentious. Here we find that both diets high in the fermentable fiber inulin and vertical sleeve gastrectomy increase intestinal expression and circulating levels of the anti-microbial peptide Reg3g. Moreover, a number of beneficial effects of these manipulations on gut function, energy balance, and glucose regulation are absent in Reg3g knockout mice. Peripheral administration of various preparations of Reg3g improves glucose tolerance, and this effect is dependent on the putative receptor Extl3 in the pancreas. These data suggest Reg3g acts both within the lumen and as a gut hormone to link the intestinal microbiome to various aspects of host physiology that may be leveraged for novel treatment strategies.

Keywords: Reg3g, obesity, type 2 diabetes, gut function, gut microbiome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Diabetes Mellitus, Type 2*
  • Glucose / metabolism
  • Intestine, Small / metabolism
  • Mice
  • Mice, Knockout
  • Microbiota*
  • N-Acetylglucosaminyltransferases
  • Pancreatitis-Associated Proteins
  • Peptides

Substances

  • Glucose
  • Peptides
  • Extl3 protein, mouse
  • N-Acetylglucosaminyltransferases
  • Reg3g protein, mouse
  • Pancreatitis-Associated Proteins