Identification and characterization of bluetongue virus in Culicoides spp. and clinically healthy livestock in southeastern Kazakhstan

Andrey V Zhigailov; Yuliya V Perfilyeva; Elina R Maltseva; Yekaterina O Ostapchuk; Alena S Cherusheva; Dinara A Naizabayeva; Anna S Nizkorodova; Zhanna A Berdygulova; Akzhigit S Mashzhan; Akerke O Bissenbay; Saltanat A Kuatbekova; Zhumagali K Koshemetov; Nurshat Abdolla; Yuriy A Skiba; Seidigapbar M Mamadaliyev

doi:10.1016/j.cimid.2022.101895

Identification and characterization of bluetongue virus in Culicoides spp. and clinically healthy livestock in southeastern Kazakhstan

Comp Immunol Microbiol Infect Dis. 2022 Nov-Dec:90-91:101895. doi: 10.1016/j.cimid.2022.101895. Epub 2022 Oct 7.

Authors

Andrey V Zhigailov¹, Yuliya V Perfilyeva², Elina R Maltseva³, Yekaterina O Ostapchuk¹, Alena S Cherusheva⁴, Dinara A Naizabayeva⁵, Anna S Nizkorodova¹, Zhanna A Berdygulova⁴, Akzhigit S Mashzhan⁵, Akerke O Bissenbay⁴, Saltanat A Kuatbekova⁴, Zhumagali K Koshemetov⁶, Nurshat Abdolla⁷, Yuriy A Skiba³, Seidigapbar M Mamadaliyev⁴

Affiliations

¹ Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan.
² Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan. Electronic address: [email protected].
³ Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan; Tethys Scientific Society, Almaty 050063, Kazakhstan.
⁴ Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan.
⁵ Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
⁶ Research Institute for Biological Safety Problems, Gvardeyskiy, Korday District, Zhambyl Region 080409, Kazakhstan.
⁷ Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan; Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.

PMID: 36244122
DOI: 10.1016/j.cimid.2022.101895

Abstract

Objective: Bluetongue is an arthropod-borne disease of ruminants. Here, we investigated the seroprevalence of bluetongue virus (BTV) in livestock and performed the first genetic characterization of BTV isolated from sheep and Culicoides midges in the southeastern region of Kazakhstan.

Methods: Blood samples were collected from 1241 asymptomatic livestock. In addition, 497 Culicoides midges were collected. Samples were analyzed for specific anti-BTV antibodies and BTV RNA by ELISA and conventional RT-PCR, respectively.

Results: The overall seroprevalence of BTV antibodies was shown to be 4.3 % (46/1079) in small ruminant and 1 % (1/82) in cattle. Anti-BTV antibodies were not detected in camels (0/80). The minimum infection rate of BTV in Culicoides was shown to be 0.24 %. Seg-2 and Seg-10 sequence analysis demonstrated that all isolates belonged to the 'western' topotype of the BTV-9 strain.

Conclusion: The present data confirm circulation of BTV in southeastern Kazakhstan.

Keywords: Bluetongue; Culicoides; Kazakhstan; Livestock; Serotype; Virus.

MeSH terms

Animals
Antibodies, Viral
Bluetongue virus* / genetics
Cattle
Ceratopogonidae*
Kazakhstan / epidemiology
Livestock
Seroepidemiologic Studies
Sheep

Substances

Antibodies, Viral