Clinical indocyanine green-based silk fibroin theranostic nanoprobes for in vivo NIR-I/II fluorescence imaging of cervical diseases

Rong Ma; Xiaohui Tang; Mei Wang; Zhong Du; Shuang Chen; Youqiang Heng; Lijun Zhu; Nuernisha Alifu; Xueliang Zhang; Cailing Ma

doi:10.1016/j.nano.2022.102615

Clinical indocyanine green-based silk fibroin theranostic nanoprobes for in vivo NIR-I/II fluorescence imaging of cervical diseases

Nanomedicine. 2023 Jan:47:102615. doi: 10.1016/j.nano.2022.102615. Epub 2022 Oct 18.

Authors

Rong Ma¹, Xiaohui Tang², Mei Wang², Zhong Du¹, Shuang Chen¹, Youqiang Heng¹, Lijun Zhu³, Nuernisha Alifu⁴, Xueliang Zhang⁵, Cailing Ma⁶

Affiliations

¹ Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, People's Republic of China.
² School of Pharmacy, Xinjiang Medical University, Urumqi, People's Republic of China.
³ State Key Laboratory of Pathogenesis, Prevention, and Treatment of High Incidence Diseases in Central Asia, School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, People's Republic of China.
⁴ State Key Laboratory of Pathogenesis, Prevention, and Treatment of High Incidence Diseases in Central Asia, School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, People's Republic of China. Electronic address: [email protected].
⁵ State Key Laboratory of Pathogenesis, Prevention, and Treatment of High Incidence Diseases in Central Asia, School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, People's Republic of China. Electronic address: [email protected].
⁶ Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, People's Republic of China. Electronic address: [email protected].

PMID: 36265558
DOI: 10.1016/j.nano.2022.102615

Abstract

Cervical diseases such as lymph node disease and tubal obstruction have threatened women's health. However, the traditional diagnostic methods still have shortcomings. NIR-II fluorescence imaging with advantages of low scattering, negligible autofluorescence, and high spatial resolution could be an ideal option. To obtain high quality NIR-II fluorescence imaging, selecting appropriate nanoprobes becomes the important issue. As a small molecular photothermal agent, extensive applications of ICG are rather limited because of its drawbacks. Herein, natural silk fibroin (SF) was synthesized and encapsulated ICG molecules to form SF@ICG nanoparticles (NPs). After detailed analysis, SF@ICG NPs showed excellent stability and long circulation time, as well as strong NIR-II fluorescence emission, well photo-stability, biocompatibility and well photothermal property under 808 nm laser irradiation. Furthermore, SF@ICG NPs were utilized for NIR-II fluorescence imaging of lymph node/lymphangiography and angiography of fallopian tubes. The process of fallopian tubes could be detected with high resolution and high sensitivity.

Keywords: Indocyanine green; NIR-II fluorescence imaging; Salpingography; Sentinel lymph node; Silk fibroin nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Female
Fibroins*
Humans
Indocyanine Green*
Optical Imaging

Substances

Indocyanine Green
Fibroins