CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design

Christian Anthon; Giulia Ilaria Corsi; Jan Gorodkin

doi:10.1093/bioinformatics/btac697

CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design

Bioinformatics. 2022 Dec 13;38(24):5437-5439. doi: 10.1093/bioinformatics/btac697.

Authors

Christian Anthon¹, Giulia Ilaria Corsi¹, Jan Gorodkin¹

Affiliation

¹ Center for Non-Coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.

Abstract

Summary: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.

Availability and implementation: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Gene Editing
RNA, Guide, CRISPR-Cas Systems*

Substances

RNA, Guide, CRISPR-Cas Systems

Abstract

Publication types

MeSH terms

Substances

Grants and funding