A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls

Konstantinos Sousounis; Katharine Courtemanche; Jessica L Whited

doi:10.1007/978-1-0716-2659-7_22

A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls

Methods Mol Biol. 2023:2562:335-349. doi: 10.1007/978-1-0716-2659-7_22.

Authors

Konstantinos Sousounis¹, Katharine Courtemanche¹, Jessica L Whited^{2

3

4}

Affiliations

¹ Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
² Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. [email protected].
³ The Harvard Stem Cell Institute, Cambridge, MA, USA. [email protected].
⁴ The Broad Institute of MIT and Harvard, Cambridge, MA, USA. [email protected].

PMID: 36272086
DOI: 10.1007/978-1-0716-2659-7_22

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.

Keywords: Axolotl screening; Axolotls; CRISPR; CRISPR design; CRISPR-Cas9; Genome targeting.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Ambystoma mexicanum / genetics
Animals
CRISPR-Cas Systems* / genetics
Gene Editing*
Genome
Mutation
RNA, Guide, CRISPR-Cas Systems / genetics

Substances

RNA, Guide, CRISPR-Cas Systems

Abstract

Publication types

MeSH terms

Substances

Grants and funding