Determination of basic criteria that influence the functionality of gluten protein fractions and gluten complex on roll bread characteristics

Determinations of basic criteria that influence the functionality of gluten protein fractions on roll bread characteristics were investigated. SE-HPLC and RP-HPLC methods were used to quantification of protein/gluten composition of six different wheat flour samples with unique quality characteristics. Consequently, following new findings were reached: The ratio of HMW-GS/LMW-GS is more important than the amounts of HMW-GS and LMW-GS in flour. And as this rate increases, bread characteristics improve. In determining dough resistance, bread elasticity and height; the ratio of insoluble to soluble glutenin is influential factor and this ratio should be at the level of 1.3-1.4. When the pointer changes towards to insoluble glutenin; the dough is more resistant, the bread is more elastic and heighten, but the viscosity is weaker. Best bread flour had balance not only between gliadin and glutenin; but also, soluble glutenin and insoluble glutenin (1:1). Strong flours had 25-30%, whereas weak flours had 16% insoluble glutenin, as a percentage of flour protein.