Three-dimensional (3D) deformation distribution measurement is of great interest in applications of materials evaluation. In this study, we propose a stereo sampling moiré method for full-field 3D deformation measurement based on a stereomicroscope. From the phase analysis on the left and right image planes as well as the relationship between displacements in the world and image coordinate systems, the 3D displacements and the in-plane strains of the specimen can be acquired. We also propose a method to calibrate the microscope convergence angle using the grid pitch variation. The validation experiment shows that the difference between the out-of-plane displacement measured by the proposed method and the movement of the sample stage is less than 0.2 µm. The microscopic 3D displacements and the in-plane strain distributions of a carbon fiber reinforced plastic specimen in a three-point bending test are investigated.