Prolonged electrolysis injures the neural development of zebrafish (Danio rerio)

Recently, electrolysis technology has been widely applied in nitrogen and phosphorus removal in river water due to its high efficiency, but its effects on aquatic animals, especially on their neurodevelopmental system, are still unclear. In this study, zebrafish (Danio rerio) embryos were used as model organisms and were put into an electrolytic reaction device with a Ti/IrO₂/RuO₂ mesh plate as the anode and a Ti mesh plate as the cathode to explore the effects of prolonged electrolysis on the nervous system. The neural development of zebrafish embryos was injured when the current density was greater than 0.89 A/m². Compared with the control group, the movement speed of zebrafish larvae (120 h postfertilization, hpf) was significantly reduced from 65.48 ± 23.69 to 48.08 ± 22.73 mm/min in a dark environment with an electric current density of 0.89 A/m² in the electrolysis group. In addition, the acetylcholinesterase activity of zebrafish larvae (120 hpf) gradually decreased from 7.60 ± 0.55 to 6.00 ± 0.01 U/mg prot and the dopamine concentration was reduced from 46.96 ± 0.85 to 40.86 ± 1.05 pg/mL with an electric current density from 0 to 0.89 A/m² in the electrolysis groups. Furthermore, the expression of nerve-related genes (syn2a, mbp, nestin, and AChE) was significantly inhibited when the current density was more than 0.89 A/m². However, there were few adverse effects on the neural development of zebrafish embryos when the current density was less than 0.86 A/m². Thus, a current density of 0.86 A/m² is a reference value to reduce the harm to the neural development of fish when electrolysis technology is used in river water pollutant treatment.