Molecular characterization and clinical impact of human bocavirus at a tertiary hospital in Barcelona (Catalonia, Spain) during the 2014-2017 seasons

Infection. 2023 Aug;51(4):935-943. doi: 10.1007/s15010-022-01955-z. Epub 2022 Nov 19.

Abstract

Purpose: The aim was to describe the prevalence, molecular epidemiology and clinical manifestations of human bocavirus (HBoV) in patients attended at a tertiary hospital in Barcelona, Spain.

Methods: From October 2014 to May 2017, respiratory specimens from paediatric patients were collected for respiratory viruses' laboratory-confirmation. Phylogenetic analyses from partial VP1 sequences were performed from all HBoV laboratory-confirmed specimens. Clinical features were retrospectively studied.

Results: 178/10271 cases were HBoV laboratory-confirmed. The median age was 1.53 (IQR 1.0-2.3). Co-detection was highly reported (136; 76%). All viruses belonged into HBoV1 genotype but one into HBoV2. Non-reported mutations were observed and two sites were suggestive to be under negative selection. 61% (109/178) cases had lower RTI (LRTI), of whom 84 had co-detections (77%) and 76 had comorbidities (70%). LRTI was the cause of hospitalization in 85 out of 109 cases (78%), and no differences were found regarding severity factors during hospitalization between co- and single-detections, except for median length of respiratory support, which was longer in cases with co-detections.

Conclusions: Close monitoring of predominant HBoV1 showed a high similarity between viruses. The presence of comorbidities might explain the high prevalence of LRTI. Symptomatology in HBoV single-detected cases suggest that HBoV is a true pathogen.

Keywords: Clinical impact; Human bocavirus; Molecular characterization; Surveillance.

MeSH terms

  • Child
  • Human bocavirus* / genetics
  • Humans
  • Infant
  • Parvoviridae Infections* / diagnosis
  • Parvoviridae Infections* / epidemiology
  • Phylogeny
  • Respiratory Tract Infections* / diagnosis
  • Retrospective Studies
  • Seasons
  • Spain / epidemiology
  • Tertiary Care Centers
  • Viruses*