Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Chem Commun (Camb). 2022 Dec 13;58(99):13724-13727. doi: 10.1039/d2cc04823j.

Abstract

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

MeSH terms

  • Fluorescence Resonance Energy Transfer
  • Microscopy*
  • Nanotechnology*
  • Proteins

Substances

  • Proteins