Chromosome conformation capture technology and its derivatives have been widely used to study genome organization. Among them, Hi-C (chromosome conformation capture coupling with high-throughput sequencing) is popular in dissecting chromatin architecture on the genome-wide level. However, the intrinsic limitations prevent its application when it comes to rare samples. Here, we present easy Hi-C, a biotin-free technology that dramatically reduces DNA loss and is suitable for low-input samples.
Keywords: Chromatin architecture; Chromosome conformation capture; Deep sequencing; Easy Hi-C; Hi-C; Proximity ligation; Re-linearization; Restriction enzyme digestion; Self-circularization.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.