Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats

Suzhen Lei; Shuqi He; Xuan Li; Baodong Zheng; Yi Zhang; Hongliang Zeng

doi:10.1016/j.foodchem.2022.134599

Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats

Food Chem. 2023 Mar 15;404(Pt A):134599. doi: 10.1016/j.foodchem.2022.134599. Epub 2022 Oct 13.

Authors

Suzhen Lei¹, Shuqi He¹, Xuan Li¹, Baodong Zheng², Yi Zhang³, Hongliang Zeng⁴

Affiliations

¹ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
³ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Subtropical Characteristic Fruits, Vegetables and Edible Fungi Processing (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China. Electronic address: [email protected].
⁴ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Subtropical Characteristic Fruits, Vegetables and Edible Fungi Processing (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China. Electronic address: [email protected].

PMID: 36444019
DOI: 10.1016/j.foodchem.2022.134599

Abstract

Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats. LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats. Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats. Furthermore, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group were positively correlated with Dehydro-LCA, isoLCA, TCA, LCA-3-S, TCHO, TG and LDL-C. In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.

Keywords: Bile acids; Hyperlipidemic rats; Lotus seed resistant starch; Microbial mechanism; Small intestinal flora.

MeSH terms

Animals
Bacteroidetes
Bile Acids and Salts
Clostridiales
Eubacterium
Gastrointestinal Microbiome / drug effects
Hyperlipidemias* / microbiology
Hyperlipidemias* / therapy
Lotus*
Rats
Resistant Starch* / pharmacology
Seeds* / chemistry

Substances

Bile Acids and Salts
Resistant Starch

Supplementary concepts

Eubacterium coprostanoligenes