Nanostructured electrodes based on multiwalled carbon nanotube/glyconanoparticles for the specific immobilization of bilirubin oxidase: Application to the electrocatalytic O₂ reduction

Here we describe the design and the characterization of novel electrode materials consisting of multi-walled carbon nanotubes coated with glyconanoparticles (GNPs) functionalized with anthraquinone sulfonate. The resulting modified electrodes were characterized by scanning electron microscopy and cyclic voltammetry. Their electrochemical behavior reveals a stable pH-dependent redox signal characteristic of anthraquinone sulfonate. Immobilization of bilirubin oxidase on these three-dimensional electrodes leads to the electroenzymatic reduction of O₂ to water with an onset potential of 0.5 V/SCE (saturated calomel electrode). A catalytic cathodic current of 174 µA (0.88 mA cm^-2) at 0.1 V/SCE, demonstrates that glyconanoparticles modified by anthraquinone sulfonate were able to interact and orientate bilirubin oxidase by electrostatic interactions.