Multisite Detection of Tn 1549-Mediated vanB Vancomycin Resistance in Multidrug-Resistant Enterococcus faecalis ST6 in Texas and Florida

Shelby R Simar; Truc T Tran; Kirsten B Rydell; Diana Panesso; German A Contreras; Jose M Munita; Renzo O Cifuentes; Lilian M Abbo; Pranoti Sahasrabhojane; An Q Dinh; Dierdre B Axell-House; Tor Savidge; Samuel A Shelburne; Blake M Hanson; Cesar A Arias

doi:10.1128/aac.01284-22

Multisite Detection of Tn 1549-Mediated vanB Vancomycin Resistance in Multidrug-Resistant Enterococcus faecalis ST6 in Texas and Florida

Antimicrob Agents Chemother. 2023 Jan 24;67(1):e0128422. doi: 10.1128/aac.01284-22. Epub 2022 Dec 21.

Authors

Shelby R Simar¹, Truc T Tran^{2

3}, Kirsten B Rydell^{2

3}, Diana Panesso^{2

3

4}, German A Contreras⁵, Jose M Munita^{6

7}, Renzo O Cifuentes⁸, Lilian M Abbo⁸, Pranoti Sahasrabhojane⁹, An Q Dinh^{2

3}, Dierdre B Axell-House^{2

3}, Tor Savidge¹⁰, Samuel A Shelburne⁹, Blake M Hanson¹, Cesar A Arias^{2

3

11}

Affiliations

¹ Center for Infectious Diseases, University of Texas Health Science Center, School of Public Health, Houston, Texas, USA.
² Division of Infectious Diseases, Houston Methodist Hospital, Houston, Texas, USA.
³ Center for Infectious Diseases, Houston Methodist Research Institute, Houston, Texas, USA.
⁴ Molecular Genetics and Antimicrobial Resistance Unit, International Center for Microbial Genomics, Universidad El Bosque, Bogotá, Colombia.
⁵ Division of Infectious Diseases, Department of Internal Medicine, McGovern Medical School, University of Texas Health Science Center, Houston, Texas, USA.
⁶ Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina Clínica Alemana de Santiago, Universidad del Desarrollo, Santiago, Chile.
⁷ Millennium Initiative for Collaborative Research On Bacterial Resistance (MICROB-R), Santiago, Chile.
⁸ Jackson Health System, Miami Transplant Institute, Miami, Florida, USA.
⁹ Department of Infectious Diseases, Division of Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
¹⁰ Texas Children's Microbiome Center, Department of Pathology, Texas Children's Hospital, Houston, Texas, USA.
¹¹ Department of Medicine, Weill Cornell Medical College, New York, New York, USA.

Abstract

In the United States, vanB-mediated resistance in enterococci is rare. We characterized three sequence type (ST) 6, vancomycin-resistant Enterococcus faecalis isolates causing bacteremia in unique patients in spatiotemporally distinct settings. Isolates were recovered between 2018 and 2020 in two cities in the United States (Houston, TX; Miami, FL). The isolates harbored the vanB operon on a chromosomally located Tn1549 transposon, and epidemiological data suggested multiple introductions of the vanB gene cluster into ST6 E. faecalis.

Keywords: Enterococcus; VRE; antimicrobial resistance.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Enterococcus faecalis / genetics
Enterococcus faecium*
Florida / epidemiology
Gram-Positive Bacterial Infections* / drug therapy
Gram-Positive Bacterial Infections* / epidemiology
Humans
Texas / epidemiology
Vancomycin Resistance / genetics
Vancomycin-Resistant Enterococci* / genetics

Substances

Bacterial Proteins
Anti-Bacterial Agents

Abstract

Publication types

MeSH terms

Substances

Grants and funding