MicroRNAs within the Basal-like signature of Quadruple Negative Breast Cancer impact overall survival in African Americans

Anusha Angajala; Hughley Raymond; Aliyu Muhammad; Md Shakir Uddin Ahmed; Saadia Haleema; Monira Haque; Honghe Wang; Moray Campbell; Rachel Martini; Balasubramanian Karanam; Andrea G Kahn; Deepa Bedi; Melissa Davis; Ming Tan; Windy Dean-Colomb; Clayton Yates

doi:10.1038/s41598-022-26000-9

MicroRNAs within the Basal-like signature of Quadruple Negative Breast Cancer impact overall survival in African Americans

Sci Rep. 2022 Dec 22;12(1):22178. doi: 10.1038/s41598-022-26000-9.

Authors

Anusha Angajala^{1

2}, Hughley Raymond¹, Aliyu Muhammad^{1

3}, Md Shakir Uddin Ahmed^{1

4}, Saadia Haleema², Monira Haque², Honghe Wang¹, Moray Campbell⁵, Rachel Martini⁶, Balasubramanian Karanam¹, Andrea G Kahn⁷, Deepa Bedi¹, Melissa Davis⁶, Ming Tan⁸, Windy Dean-Colomb^{1

9}, Clayton Yates^{10

11

12

13}

Affiliations

¹ Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL, 36088, USA.
² Department of Pathology, University of South Alabama, Mobile, AL, 36604, USA.
³ Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, 810107, Kaduna State, Nigeria.
⁴ Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh.
⁵ Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA.
⁶ Department of Surgery, Weill Cornell Medicine, New York, NY, 10021, USA.
⁷ Department of Pathology, The University of Alabama at Birmingham, Birmingham, AL, 35249-7331, USA.
⁸ Graduate Institute of Biomedical Sciences and Research Center for Cancer Biology, China Medical University, Taichung, 406040, Taiwan.
⁹ Department of Hematology/Oncology, Piedmont Hospital, Newnan, GA, 30265, USA.
¹⁰ Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL, 36088, USA. [email protected].
¹¹ Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, 21218, USA. [email protected].
¹² Cancer Genetics and Epigenetics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, The Bunting-Blaustein Cancer Research Building 1, 1650 Orleans Street - Room 1M44, Baltimore, MD, 21287-0013, USA. [email protected].
¹³ Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, 21218, USA. [email protected].

Abstract

We previously found that QNBC tumors are more frequent in African Americans compared to TNBC tumors. To characterize this subtype further, we sought to determine the miRNA-mRNA profile in QNBC patients based on race. Both miRNA and mRNA expression data were analyzed from TCGA and validated using datasets from the METABRIC, TCGA proteomic, and survival analysis by KMPLOT. miRNA-mRNAs which include FOXA1 and MYC (mir-17/20a targets); GATA3 and CCNG2 (mir-135b targets); CDKN2A, CDK6, and B7-H3 (mir-29c targets); and RUNX3, KLF5, IL1-β, and CTNNB1 (mir-375 targets) were correlated with basal-like and immune subtypes in QNBC patients and associated with a worse survival. Thus, QNBC tumors have an altered gene signature implicated in racial disparity and poor survival.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Black or African American / genetics
Breast Neoplasms* / genetics
Female
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
Proteomics
RNA, Messenger
Triple Negative Breast Neoplasms* / pathology

Substances

MicroRNAs
RNA, Messenger

Abstract

Publication types

MeSH terms

Substances

Grants and funding