Comparative study of zinc oxide nanoparticles synthesized through biogenic and chemical route with reference to antibacterial, antibiofilm and anticancer activities

Nallasamy Prakashkumar; Arivalagan Pugazhendhi; Kathirvel Brindhadevi; Hakim Al Garalleh; Mazen Garaleh; Natarajan Suganthy

doi:10.1016/j.envres.2022.115136

Comparative study of zinc oxide nanoparticles synthesized through biogenic and chemical route with reference to antibacterial, antibiofilm and anticancer activities

Environ Res. 2023 Mar 1:220:115136. doi: 10.1016/j.envres.2022.115136. Epub 2022 Dec 28.

Authors

Nallasamy Prakashkumar¹, Arivalagan Pugazhendhi², Kathirvel Brindhadevi³, Hakim Al Garalleh⁴, Mazen Garaleh⁵, Natarajan Suganthy⁶

Affiliations

¹ Department of Nanoscience and Technology, Alagappa University, Karaikudi, Tamil Nadu, India.
² School of Engineering, Lebanese American University, Byblos, Lebanon; University Centre for Research & Development, Department of Civil Engineering, Chandigarh University, Mohali, India. Electronic address: [email protected].
³ Center for Transdisciplinary Research (CFTR), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
⁴ Department of Mathematical Science, College of Engineering, University of Business and Technology-Dahban, Jeddah, 21361, Saudi Arabia.
⁵ Department of Mathematical Science, College of Engineering, University of Business and Technology-Dahban, Jeddah, 21361, Saudi Arabia; Department of Applied Chemistry, Faculty of Science, Tafila Technical University, Tafila, 66141, Jordan.
⁶ Department of Nanoscience and Technology, Alagappa University, Karaikudi, Tamil Nadu, India. Electronic address: [email protected].

PMID: 36584851
DOI: 10.1016/j.envres.2022.115136

Abstract

The present focused on comparative study on synthesis of ZnO nanoparticles (ZnO NPs) using chemical method via alkaline precipitation method (ZnO(A) NPs) using NaOH and biogenic method using termite mound extract (ZnO(B) NPs). GC-MS analysis revealed that D-limonene present in termite mound extract might be responsible for the synthesis of ZnO(B) NPs. XRD patterns confirmed hexagonal crystalline structure of ZnO(A) and (B) NPs. Results of antibacterial activity illustrated that ZnO(B) NPs showed its potential against Pseudomonas aeruginosa, ESBL-1, ESBL-2 and EBSL-3. Antibiofilm studies revealed that ZnO(B) NPs exhibited optimum decline in MRSA biofilm formation than ZnO(A) NPs. In addition, ZnO(B) NPs showed potent cytotoxic effect against lung cancer cell lines A549 with IC₅₀ of 35.16 ± 0.10 μg/mL in comparison with ZnO(A) NPs (IC_50- 55.09 ± 0.30 μg/mL). Overall, the results revealed that biogenic synthesis of ZnO NPs ensures its biosafety level and enhanced biological activity when compared to chemical synthesis method.

Keywords: Antibacterial activity; Antibiofilm efficiency; Anticancer potential; D-limonene; Termite mound extract; Zinc oxide NPs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Biofilms
Metal Nanoparticles* / chemistry
Microbial Sensitivity Tests
Plant Extracts / chemistry
Zinc Oxide* / chemistry
Zinc Oxide* / pharmacology

Substances

Zinc Oxide
Anti-Bacterial Agents
Plant Extracts