A new approach of magnetic field application in miniaturized pipette-tip extraction for trace analysis of four synthetic hormones in breast milk samples

Herein, a novel homemade electrical device was designed, including two pieces of external neodymium magnets, providing a reciprocating magnetic field to introduce a magnetic-assisted dispersive pipette-tip micro solid-phase extraction. To evaluate the performance efficiency of the proposed method, a novel magnetic calcined GO/SiO₂@Co-Fe nanocube sorbent was synthesized, filled into the pipette-tip, exposed to the reciprocating magnetic field, and applied for the preconcentration of some hormone therapy drugs in human biological matrices. The effective adsorption and desorption parameters were optimized using a rotatable central composite design and one-variable-at-a-time approaches. Under the optimized conditions, the target analytes' detection limits were found to be below 0.02 ng mL^-1. Moreover, the calibration curves were linear in the range of 0.03-500.00 ng mL^-1 (R² > 0.9966), with RSDs% less than 7.8 %. Eventually, the established method was applied to extract the analytes from breast milk samples, followed by LC-ESI-MS/MS analysis.