Mass Spectrometry of Neutral Glycosphingolipids

Akemi Suzuki; Atit Silsirivanit; Takashi Watanabe; Junko Matsuda; Kei-Ichiro Inamori; Jin-Ichi Inokuchi

doi:10.1007/978-1-0716-2910-9_11

Mass Spectrometry of Neutral Glycosphingolipids

Methods Mol Biol. 2023:2613:127-144. doi: 10.1007/978-1-0716-2910-9_11.

Authors

Akemi Suzuki¹, Atit Silsirivanit², Takashi Watanabe³, Junko Matsuda³, Kei-Ichiro Inamori⁴, Jin-Ichi Inokuchi⁴

Affiliations

¹ Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan. [email protected].
² Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
³ Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
⁴ Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.

PMID: 36587076
DOI: 10.1007/978-1-0716-2910-9_11

Abstract

This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.

Keywords: Ceramide structures; Glycan sequences; Glycosphingolipids (GSLs); Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS); Mass spectrometry (MS); Multiple reaction monitoring (MRM); Reversed phase liquid chromatography; Thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS).

MeSH terms

Chromatography, Liquid
Chromatography, Thin Layer / methods
Glycosphingolipids / chemistry
Neutral Glycosphingolipids* / chemistry
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Neutral Glycosphingolipids
Glycosphingolipids