Thermal optimum of photosynthesis is controlled by stomatal conductance and does not acclimate across an urban thermal gradient in six subtropical tree species

Modelling the response of plants to climate change is limited by our incomplete understanding of the component processes of photosynthesis and their temperature responses within and among species. For ≥20 individuals, each of six common subtropical tree species occurring across steep urban thermal gradients in Miami, Florida, USA, we determined rates of net photosynthesis (A_net ), maximum RuBP carboxylation, maximum RuBP regeneration and stomatal conductance, and modelled the optimum temperature (T_opt ) and process rate of each parameter to address two questions: (1) Do the T_opt of A_net (T_optA ) and the maximum A_net (A_opt ) of subtropical trees reflect acclimation to elevated growth temperatures? And (2) What limits A_net in subtropical trees? Against expectations, we did not find significant acclimation of T_optA , A_opt or the T_opt of any of the underlying photosynthetic parameters to growth temperature in any of the focal species. Model selection for the single best predictor of A_net both across leaf temperatures and at T_optA revealed that the A_net of most trees was best predicted by stomatal conductance. Our findings are in accord with those of previous studies, especially in the tropics, that have identified stomatal conductance to be the most important factor limiting A_net , rather than biochemical thermal responses.