Effect of phosphorylation in vitro of human fibrinogen with protein kinase C on thrombin-induced gelation

Thromb Res. 1987 Jul 1;47(1):93-9. doi: 10.1016/0049-3848(87)90244-1.

Abstract

Thrombin-induced gel formation of fibrinogen phosphorylated by protein kinase C yielded a transparent gel, whereas unphosphorylated fibrinogen yielded a coarse gel. The mass-length ratio was found to be one order of magnitude higher for the unphosphorylated than for the phosphorylated fibrinogen. Since the phosphorylated sites are located near the cross-linking sites in the A alpha-chain of fibrinogen, it is likely that the introduction of charged phosphate groups in this region prevent the lateral growth of the fibrin fibres.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Fibrin / metabolism
  • Fibrinogen / metabolism*
  • Fibrinogen / physiology
  • Fibrinopeptide A / metabolism
  • Fibrinopeptide B / metabolism
  • Humans
  • Phosphorylation
  • Protein Conformation
  • Protein Kinase C / pharmacology*
  • Thrombin / pharmacology*
  • Time Factors

Substances

  • Fibrinopeptide A
  • Fibrinopeptide B
  • Fibrin
  • Fibrinogen
  • Protein Kinase C
  • Thrombin