A novel electrochemical biosensor based on AMNFs@ZIF-67 nano composite material for ultrasensitive detection of HER2

Ya Zhang; Ning Li; Ying Xu; Xiaofang Liu; Yi Ma; Zhen Huang; Huibo Luo; Changjun Hou; Danqun Huo

doi:10.1016/j.bioelechem.2022.108362

A novel electrochemical biosensor based on AMNFs@ZIF-67 nano composite material for ultrasensitive detection of HER2

Bioelectrochemistry. 2023 Apr:150:108362. doi: 10.1016/j.bioelechem.2022.108362. Epub 2022 Dec 30.

Authors

Ya Zhang¹, Ning Li¹, Ying Xu¹, Xiaofang Liu¹, Yi Ma², Zhen Huang³, Huibo Luo², Changjun Hou⁴, Danqun Huo⁵

Affiliations

¹ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China.
² Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, 188 University Town, Yi bin 644000, PR China.
³ Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, SeNA Research Institute and Szostak-CDHT Large Nucleic Acids Institute, Chengdu, 610000, PR China.
⁴ Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, 188 University Town, Yi bin 644000, PR China. Electronic address: [email protected].
⁵ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing 400044, PR China. Electronic address: [email protected].

PMID: 36608370
DOI: 10.1016/j.bioelechem.2022.108362

Abstract

Antimonene (AMNFs) is a new kind of sp²-bonded honeycomb lattice two-dimensional material with strong rod-rail coupling, stability and hydrophilicity. Compared with graphene, antimonene has better sensitivity in DNA molecular sensing. We developed a suitable biosensor-Cd²⁺-aptamer@AMNFs@ZIF-67 nanocomposite for HER2 biomarker detection. Two-dimensional antimonene can be grown on the surface of ZIF-67 to enhance stability and biocompatibility. The aptamer chain can also be adsorbed on the surface of antimonene, and the complexes of the aptamer and the marker can be detached analytically after targeting the biomarker. The detection limit (LOD) was 4.853 fg/mL within 60 min, the detection range was 0-1000 pg/mL, and the LOD was lower than the existing HER2 aptamer biosensors. The results show that the biosensor has certain applicability and potential, and is expected to be a powerful tool for breast cancer diagnosis in the future.

Keywords: AMNFs; Electrochemical aptasensor; HER2; ZIF-67.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Electrodes
Gold / chemistry
Humans
Limit of Detection
Nanocomposites* / chemistry

Substances

Aptamers, Nucleotide
Gold