New iridoids from Patrinia scabiosaefolia and their hypoglycemic effects by activating PI3K/Akt signaling pathway

Zhenhua Liu; Lijun Meng; Mengke Wang; Li Wang; Yuhang Liu; Gaixia Hou; Shiming Li; Wenyi Kang

doi:10.1016/j.fitote.2022.105423

New iridoids from Patrinia scabiosaefolia and their hypoglycemic effects by activating PI3K/Akt signaling pathway

Fitoterapia. 2023 Mar:165:105423. doi: 10.1016/j.fitote.2022.105423. Epub 2023 Jan 3.

Authors

Zhenhua Liu¹, Lijun Meng², Mengke Wang², Li Wang³, Yuhang Liu³, Gaixia Hou⁴, Shiming Li⁵, Wenyi Kang⁶

Affiliations

¹ National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Shenzhen Research Institute of Henan University, Shenzhen 518000, China.
² National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Kaifeng 475004, China.
³ National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, China.
⁴ National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; College of Physical Education, Henan University, Kaifeng 475004, China. Electronic address: [email protected].
⁵ National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China. Electronic address: [email protected].
⁶ National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Shenzhen Research Institute of Henan University, Shenzhen 518000, China; Functional Food Engineering Technology Research Center, Kaifeng 475004, China. Electronic address: [email protected].

PMID: 36608711
DOI: 10.1016/j.fitote.2022.105423

Abstract

Growing in regions of Asia and North America, Patrinia scabiosaefolia is a wild vegetable and herb that has demonstrated health-promoting properties. Iridoids are one of the most bioactive phytochemicals in P. scabiosaefolia but the in-depth study is scarce. Herein we reported the separation and characterization of nine iridoids (compounds 1-9) from P. scabiosaefolia, and two compounds (2 and 6) were new. All the structures of the nine iridoids were characterized and confirmed with NMR (1D & 2D), HRMS, IR and UV. Compound 2 is a five-member ring iridoid, reminiscent of a broken C-1 and C-2 bond. Compound 6 has a typical monoene valerian iridoid, but the 5-deoxyglucose moiety at C-11 position is uncommon in this genus. The anti-diabetic evaluation of the isolated compounds revealed that compounds 1, 2, and 9 significantly increased the glucose absorption in 3 T3-L1 cells (P < 0.01). Further mechanism investigations have demonstrated that compound 1 promoted glucose uptake in dexamethasone-treated 3 T3-L1 adipocytes by activating PI3K/Akt signaling pathway. The expression of GLUT4 mRNA and protein was also upregulated. These results provide scientific references for the potential use of P. scabiosaefolia as a functional food to manage hyperglycemia.

Keywords: Iridoid; PI3K/Akt; Patrinia scabiosaefolia; Patrinoside G; Patrinoside H.

MeSH terms

Hypoglycemic Agents / pharmacology
Iridoids*
Molecular Structure
Patrinia* / chemistry
Patrinia* / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Signal Transduction

Substances

Iridoids
Proto-Oncogene Proteins c-akt
Phosphatidylinositol 3-Kinases
Hypoglycemic Agents