A novel NMDA receptor test model based on hiPSC-derived neural cells

Biol Chem. 2023 Jan 12;404(4):267-277. doi: 10.1515/hsz-2022-0216. Print 2023 Mar 28.

Abstract

N-Methyl-D-aspartate receptors (NMDARs) are central for learning and information processing in the brain. Dysfunction of NMDARs can play a key role in the pathogenesis of neurodegeneration and drug addiction. The development of selective NMDAR modulators represents a promising strategy to target these diseases. Among such modulating compounds are ifenprodil and its 3-benzazepine derivatives. Classically, the effects of these NMDAR modulators have been tested by techniques like two-electrode voltage clamp (TEVC), patch clamp, or fluorescence-based assays. However, testing their functional effects in complex human systems requires more advanced approaches. Here, we established a human induced pluripotent stem cell-derived (hiPSC-derived) neural cell system and proved its eligibility as a test system for investigating NMDAR modulators and pharmaceutical effects on human neurons.

Keywords: GluN2B; NMDAR; glutamatergic neurons; human stem cells; neurology; pharmacology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Induced Pluripotent Stem Cells*
  • Neurons
  • Receptors, N-Methyl-D-Aspartate*

Substances

  • Receptors, N-Methyl-D-Aspartate