Upregulation of PTPRC and Interferon Response Pathways in HIV-1 Seroconverters Prior to Infection

Yunqi Li; Francois Lefebvre; Edith Nakku-Joloba; Allan Ronald; Glenda Gray; Guy de Bruyn; James Kiarie; Connie Celum; Mark J Cameron; Jairam R Lingappa; Romel D Mackelprang

doi:10.1093/infdis/jiac498

Upregulation of PTPRC and Interferon Response Pathways in HIV-1 Seroconverters Prior to Infection

J Infect Dis. 2023 Mar 1;227(5):714-719. doi: 10.1093/infdis/jiac498.

Authors

Yunqi Li¹, Francois Lefebvre², Edith Nakku-Joloba³, Allan Ronald⁴, Glenda Gray⁵, Guy de Bruyn⁵, James Kiarie^{6

7}, Connie Celum^{7

8

9}, Mark J Cameron¹⁰, Jairam R Lingappa^{7

8

11}, Romel D Mackelprang^{7

12}

Affiliations

¹ Institute for Public Health Genetics, University of Washington, Seattle, Washington, USA.
² Canadian Centre for Computational Genomics-Montréal Node, Montreal, Quebec, Canada.
³ School of Public Health, Makerere University, Kampala, Uganda.
⁴ Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada.
⁵ Perinatal HIV Research Unit, University of the Witwatersrand, Johannesburg, South Africa.
⁶ Department of Obstetrics and Gynecology, University of Nairobi, Nairobi, Kenya.
⁷ Department of Global Health, University of Washington, Seattle, Washington, USA.
⁸ Department of Medicine, University of Washington, Seattle, Washington, USA.
⁹ Department of Epidemiology, University of Washington, Seattle, Washington, USA.
¹⁰ Department of Population and Quantitative Health Science, Case Western Reserve University, Cleveland, Ohio, USA.
¹¹ Department of Pediatrics, University of Washington, Seattle, Washington, USA.
¹² Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.

Abstract

Human immunodeficiency virus 1 (HIV-1) exposed seronegative (HESN) individuals may have unique characteristics that alter susceptibility to HIV-1 infection. However, identifying truly exposed HESN is challenging. We utilized stored data and biospecimens from HIV-1 serodifferent couple cohorts, in which couples' HIV-1 exposures were quantified based on unprotected sex frequency and viral load of the partner with HIV-1. We compared peripheral blood gene expression between 15 HESN and 18 seroconverters prior to infection. We found PTPRC (encoding CD45 antigen) and interferon-response pathways had significantly higher expression among individuals who went on to become seropositive and thus may be a signature for increased acquisition risk.

Keywords: HIV-1; acquisition; gene expression; host resistance; microarray.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

HIV Infections*
HIV-1*
Humans
Interferons / genetics
Leukocyte Common Antigens
Up-Regulation

Substances

Interferons
Leukocyte Common Antigens
PTPRC protein, human

Abstract

Publication types

MeSH terms

Substances

Grants and funding