Lachnochromonin, a fungal metabolite from Lachnum virgineum, inhibits cell growth and promotes apoptosis in tumor cells through JAK/STAT3 signaling

Jun Chu; Wanli Zhai; Yongtao Geng; Yarui Feng; Jiayu Wang; Jun Li; Yinyin Wang; Wenying Zhuang; Yongsheng Che; Yi Li; Zhijie Chang; Fangli Ren

doi:10.1016/j.cellsig.2023.110592

Lachnochromonin, a fungal metabolite from Lachnum virgineum, inhibits cell growth and promotes apoptosis in tumor cells through JAK/STAT3 signaling

Cell Signal. 2023 Jun:106:110592. doi: 10.1016/j.cellsig.2023.110592. Epub 2023 Jan 11.

Authors

Jun Chu¹, Wanli Zhai¹, Yongtao Geng², Yarui Feng¹, Jiayu Wang¹, Jun Li¹, Yinyin Wang¹, Wenying Zhuang³, Yongsheng Che⁴, Yi Li⁵, Zhijie Chang⁶, Fangli Ren⁷

Affiliations

¹ State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Center for Synthetic and Systems Biology, Institute of Precision Medicine, Tsinghua University, Beijing 100084, China.
² State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Center for Synthetic and Systems Biology, Institute of Precision Medicine, Tsinghua University, Beijing 100084, China; Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
³ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, China.
⁴ State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China.
⁵ Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
⁶ State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Center for Synthetic and Systems Biology, Institute of Precision Medicine, Tsinghua University, Beijing 100084, China. Electronic address: [email protected].
⁷ State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Center for Synthetic and Systems Biology, Institute of Precision Medicine, Tsinghua University, Beijing 100084, China. Electronic address: [email protected].

PMID: 36641081
DOI: 10.1016/j.cellsig.2023.110592

Abstract

Natural compounds that interfere with tumor cell growth have potential to be used as therapeutic agents to treat cancers. Lachnochromonin (p71) is a small molecule isolated from Lachnum virgineum. Here, we reported the effect of p71 on human tumor cells, especially on breast cancer MCF-7 cells. We found that p71 significantly suppresses cell growth and induces apoptosis. The luciferase results demonstrated that p71 specifically attenuates the activation of JAK/STAT3 signaling. Biochemical analysis revealed that p71 blocks the phosphorylation of STAT3 tyrosine 705 and serine 727, resulting in down-regulation of c-Myc and Cyclin D1 expression level. Importantly, p71 inhibited cell growth, colony-formation, and migration through affecting STAT3 activity. These results implied that p71 may be used as a therapeutic agent against breast cancer.

Keywords: Apoptosis; Cell growth; Cell migration; Lachnochromonin; STAT3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Ascomycota
Breast Neoplasms* / pathology
Cell Line, Tumor
Cell Proliferation
Female
Humans
Phosphorylation
STAT3 Transcription Factor / metabolism
Signal Transduction

Substances

STAT3 Transcription Factor
STAT3 protein, human

Supplementary concepts

Lachnum virgineum