Co-Localization of Resistance and Metabolic Quantitative Trait Loci on Carrot Genome Reveals Fungitoxic Terpenes and Related Candidate Genes Associated with the Resistance to Alternaria dauci

Metabolites. 2023 Jan 2;13(1):71. doi: 10.3390/metabo13010071.

Abstract

Alternaria leaf blight, caused by the fungus Alternaria dauci, is the most damaging foliar disease of carrot. Some carrot genotypes exhibit partial resistance to this pathogen and resistance Quantitative Trait Loci (rQTL) have been identified. Co-localization of metabolic QTL and rQTL identified camphene, α-pinene, α-bisabolene, β-cubebene, caryophyllene, germacrene D and α-humulene as terpenes potentially involved in carrot resistance against ALB. By combining genomic and transcriptomic analyses, we identified, under the co-localization regions, terpene-related genes which are differentially expressed between a resistant and a susceptible carrot genotype. These genes include five terpene synthases and twenty transcription factors. In addition, significant mycelial growth inhibition was observed in the presence of α-humulene and caryophyllene.

Keywords: Daucus carota; antifungal activities; leaf blight; metabolomic; transcriptomic.

Grants and funding

This research was funded by a grant from Ministry for Higher Education and Research (France) and conducted in the framework of the regional program “Objectif Végétal”, Research, Education and Innovation in Pays de la Loire, supported by the French Region Pays de la Loire, Angers Loire Métropole and the European Regional Development Fund.