Nanopriming of Barley Seeds-A Shotgun Approach to Improve Germination under Salt Stress Conditions by Regulating of Reactive Oxygen Species

Abiotic stresses are the most important environmental factors affecting seed germination, and negatively affect crop production worldwide. Water availability is essential for proper seed imbibition and germination. The mechanism by which seeds can germinate in areas with high soil salinity is, however, still unclear. The present study aims to investigate the protective roles of AgNPs in alleviating stress symptoms caused by salinity exposure in barley seeds. For this purpose, different treatment combinations of seed priming with PVP-AgNPs in salinity stress conditions were used. Salt stress (150 and 200 mM) was found to reduce seed germination by 100% when compared to the control. Under NaCl concentrations, seed priming with PVP-AgNPs (40 mg L^-1) only for 2 h, reduced salinity effects. Salinity resulted in increased reactive oxygen species (ROS) generation compared to the control. However, increased antioxidants in the NPs treatments, such as SOD, CAT, GR, GPX (expression at both genes, such as HvSOD, HvCAT, HvGR or HvGPX, and protein levels) and glutathione content, scavenged these ROS. Considering all of the parameters under study, priming alleviated salt stress. To summarize, seed priming with AgNPs has the potential to alleviate salinity stress via reduced ROS generation and activation of the antioxidant enzymatic system during germination.