CircRNA mannosidase alpha class 1A member 2 promotes esophageal squamous cell carcinoma progression by regulating C-C chemokine ligand 5

Lie Liu; Meixiang Sang; Jian Shi; Yang Zheng; Lingjiao Meng; Lina Gu; Ziyi Li; Fei Liu; Jing Bu; Xiaoyang Duan; Feifei Zhao; Wentian Zhang; Baoen Shan

doi:10.1016/j.bbrc.2023.01.043

CircRNA mannosidase alpha class 1A member 2 promotes esophageal squamous cell carcinoma progression by regulating C-C chemokine ligand 5

Biochem Biophys Res Commun. 2023 Feb 19:645:61-70. doi: 10.1016/j.bbrc.2023.01.043. Epub 2023 Jan 16.

Authors

Lie Liu¹, Meixiang Sang², Jian Shi³, Yang Zheng¹, Lingjiao Meng¹, Lina Gu¹, Ziyi Li¹, Fei Liu¹, Jing Bu¹, Xiaoyang Duan³, Feifei Zhao⁴, Wentian Zhang⁵, Baoen Shan⁶

Affiliations

¹ Department of Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
² Department of Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China; Department of Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
³ Department of Medical Oncology, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
⁴ Department of Hematology, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
⁵ Department of Medical Oncology, Lingshou County Hospital of Integrated Traditional Chinese and Western Medicine, Shijiazhuang, Hebei, 050500, People's Republic of China.
⁶ Department of Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China; Department of Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China. Electronic address: [email protected].

PMID: 36680938
DOI: 10.1016/j.bbrc.2023.01.043

Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignancy with high morbidity and mortality. Although circular RNAs (circRNAs) play important roles in various cancers including ESCC, the role of the circRNA mannosidase alpha class 1A member 2 (circMAN1A2) in ESCC has been rarely studied. This study aimed to explore the role of circMAN1A2 in ESCC. CircMAN1A2 expression in ESCC tissues and cells was evaluated, and the relationship between circMAN1A2 expression and prognosis in patients with ESCC was analyzed. C-C chemokine ligand 5 (CCL5) was found to be a downstream target of circMAN1A2 by analysing the Agilent Microarray. Next, we performed in vitro and in vivo xenotransplantation assays to explore the role of circMAN1A2 in ESCC. We observed that high circMAN1A2 expression is associated with poor prognosis in patients with ESCC. Suppression of circMAN1A2 expression inhibits the proliferation, migration, and invasiveness of ESCC via regulating CCL5. Our results suggest that circMAN1A2 can promote the progression of ESCC by regulating CCL5. Thus, circMAN1A2 might be a novel diagnostic biomarker of ESCC, and targeting circMAN1A2 using inhibitors could be a potential therapeutic strategy to treat ESCC.

Keywords: CCL5; Circular RNA; Esophageal squamous cell carcinoma; circMAN1A2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Esophageal Neoplasms* / pathology
Esophageal Squamous Cell Carcinoma* / pathology
Gene Expression Regulation, Neoplastic
Humans
Ligands
Mannosidases / metabolism
MicroRNAs* / genetics
RNA, Circular / genetics
RNA, Circular / metabolism

Substances

RNA, Circular
Ligands
Mannosidases
MicroRNAs